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The Adult Immune Response Study

Probiotic supplement consumption alters cytokine production from peripheral blood mononuclear cells: a preliminary study using healthy individuals

Aim

This study examined the effect of a 12-week Lab4 probiotic and fructooligosaccharide supplementation on ex-vivo pro-inflammatory and anti-inflammatory cytokine production by peripheral blood mononuclear cells extracted from healthy volunteers.

Method

  • 20 healthy volunteers received a supplement comprising 25 billion Lab4 probiotics and 2g of fructooligosaccharide (FOS) daily for 12 weeks.
  • Blood samples were collected at the start, 6 and 12 weeks.
  • Peripheral blood mononuclear cells (PBMCs) were isolated from the blood samples and cultured ex-vivo in the absence (‘resting’) or presence (challenge) of lipopolysaccharide (bacterial toxin).
  • Results

    In the ‘resting’ immune state:
    • Significant enhancement of production of the anti-inflammatory cytokines IL-10 and TGF-β over the 12 week period (**P<0.0001)

    Fold change in IL-10 level

    Fold change in IL-1β level - part of the Adult Immune Response study
    Fold change in IL-1β level - part of the Adult Immune Response study

    Fold change in TGF-β level

    Fold change in IL-1β level - part of the Adult Immune Response study
    Fold change in IL-1β level - part of the Adult Immune Response study
    In the ‘resting’ immune state during challenge with bacterial toxin: :
    • Significant decrease in production of the pro-inflammatory cytokines IL-6 and IL-1β(*P<0.01,**P<0.0001)

    Fold change in IL-6 level

    Fold change in IL-1β level - part of the Adult Immune Response study
    Fold change in IL-1β level - part of the Adult Immune Response study
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